Autoradiographic visualization of the mouse egg's sperm receptor bound to sperm.

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Autoradiographic Visualization of the Mouse Egg's Sperm Receptor Bound to Sperm

The extracellular coat, or zona pellucida, of mammal ian eggs contains species-specific receptors to which sperm bind as a prelude to fertilization. In mice, ZP3, one of only three zona pellucida glycoproreins, serves as sperm receptor. Acrosome-intact, but not acrosome-reacted, mouse sperm recognize and interact with specific O-linked oligosaccharides of ZP3 resulting in sperm-egg binding. Bin...

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Autoradiographic visualization of the mouse egg's sperm receptor bound to sperm

The extracellular coat, or zona pellucida, of mammalian eggs contains species-specific receptors to which sperm bind as a prelude to fertilization. In mice, ZP3, one of only three zona pellucida glycoproteins, serves as sperm receptor. Acrosome-intact, but not acrosome-reacted, mouse sperm recognize and interact with specific O-linked oligosaccharides of ZP3 resulting in sperm-egg binding. Bind...

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Sperm and scrambled eggs

A newspaper obituary not long ago revealed that its subject was believed to have been both the son and grandson of the lecherous monarch, Edward VII. (Work it out for yourself.) But this is as nothing to the conundrums that Lee Silver sets out in his mesmerizing vision of our genetic future. Within weeks of the eruption of Dolly the sheep into the pages of Nature — and regardless of the doubts ...

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Direct visualization of sperm competition and sperm storage in Drosophila

Drosophila females engage in multiple matings [1] [2] [3] [4] even though they can store sperm in specialized organs for most of their life [5]. The existence of sperm competition in Drosophila has been inferred from the proportion of progeny sired by the second male in double-mating experiments [6] [7] [8]. Investigators have used this approach to quantify genetic variation underlying sperm co...

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Autoradiographic investigation of sperm transit through the male mouse genital tract after tritiated thymidine incorporation.

The transit of spermatozoa in the genital tract of the male mouse was investigated by quantitative light microscopic autoradiography after intraperitoneal injection of tritiated thymidine. Transit duration in the caput and the corpus of the epididymis was shown to be 3 days; the total duration of transit in the genital tract was 5 days. These findings indicate that the time required for the tra...

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ژورنال

عنوان ژورنال: The Journal of Cell Biology

سال: 1986

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.102.4.1363